Issue |
J. Eur. Opt. Soc.-Rapid Publ.
Volume 6, 2011
|
|
---|---|---|
Article Number | 11045 | |
Number of page(s) | 8 | |
DOI | https://doi.org/10.2971/jeos.2011.11045 | |
Published online | 07 September 2011 |
Regular papers
Self calibration of sensorless adaptive optical microscopes
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
Received:
30
May
2011
We present a self-calibrating scheme for microscopes using model-based wavefront sensorless adaptive optics. Unlike previous methods, this scheme permits the calibration of system aberration modes without the need for a separate wavefront sensor or interferometer. Basis modes are derived from the deformable mirror influence functions and an image cross-correlation method is used to remove image displacement effects from these modes. Image based measurements are used to derive an optimum modal representation from the displacement-free basis modes. These new modes are insensitive to system misalignments and the shape of the illumination profile. We demonstrate the effectiveness and robustness of these optimal modes in a third harmonic generation (THG) microscope.
Key words: Adaptive optics / aberrations / microscopy
© The Author(s) 2011. All rights reserved.
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