a) Measured localization errors for different measurement statistics. b–d) Extracted lateral target positions of 100 repetitions with 1,250 (b), 5,000 (c) and 50,000 (d) measurements per reconstruction. Histograms show the distribution of extracted target positions for a single illumination (top and right, blue histograms) and superposed reconstructions (bottom and left, yellow histograms) of each lateral axis. The black cross indicates the actual target position.