Open Access
Table 1
Set of parameters adjusted for STED microscopy in practice.
| Parameters | Definition |
|---|---|
| Pixel size | Dimension of each pixel of the image |
| Pinhole size | 1 A.U. |
| Dwell time | The time during that light is detected from each volume element |
| Line accumulation | Repeating each line including several pixels (# pixels) |
| Excitation power % | Reported based on percentage of Excitation laser power |
| STED power % | Reported based on percentage of STED laser power |
| Excitation wavelength | 561 nm |
| STED wavelength | 775 nm |
| Frame size | Dimension of each image |
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