Figure 3


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(A) SERS spectra of SS solution at increasing concentrations of lactate (0.01%, 0.1%, 0.2%, 0.4%, 0.8%, 1.5% (w/v)) acquired at three fixed pH values (black lines pH = 4.8, red lines pH = 6.6, blue lines pH = 9.0). The experiments were repeated three times on different AgNWs spots. The spectra reported represent the average of the three repetitions and were stacked for clarity. (B) Calibration curve obtained integrating the spectral raw data of the lactate vibrational band in the range 813–875 cm−1 for each concentration value in three different experiments and for three pH values. Experimental data (scatter points) exhibit a sigmoidal behaviour (coloured lines). When reported in a log–log plot (inset) the data follow a linear model (y = 4.72 + 0.30x, r 2 = 0.98 at pH 4.8; y = 4.51 + 0.39x, r 2 = 0.99 at pH 6.6 and 9.0).

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