Figure 4

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Signal of the QPD in the xy plane for a displacement of the bead of 300 nm, versus trapped-bead height. The height is calculated from the number N of dark fringes observed from contact to the slide as h = ND + R, with D = 402 nm and R = 1.5 μm. The values obtained using the objective with a phase contrast ring (blue) or without (red) are compared.

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