| Issue |
J. Eur. Opt. Society-Rapid Publ.
Volume 22, Number 1, 2026
|
|
|---|---|---|
| Article Number | 10 | |
| Number of page(s) | 13 | |
| DOI | https://doi.org/10.1051/jeos/2026005 | |
| Published online | 20 February 2026 | |
Review Article
Research on biological detection technology based on fluorescence principles
1
Shandong Laboratory of Advanced Biomaterials and Medical Devices in Weihai, Shandong 264200, PR China
2
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou 215163, PR China
* Corresponding authors: This email address is being protected from spambots. You need JavaScript enabled to view it.
(Maojie Jiang); This email address is being protected from spambots. You need JavaScript enabled to view it.
(Fuqiang Ma)
Received:
16
December
2025
Accepted:
16
January
2026
Abstract
Biological detection technology plays an important role in the fields of disease diagnosis, environmental monitoring and food safety. Among them, detection technology based on optical principle has the advantages of high sensitivity, fast response and non-destructive, which has led to its rapid development. In this review, we systematically review the principles, progress and applications of fluorescence detection, which achieves ultrasensitive detection and dynamic monitoring of biomolecules through the strategies of fluorescence labeling, fluorescence lifetime imaging, fluorescence polarization immunoassay and fluorescence resonance energy transfer. In the future, the combination of multiple technologies, intelligent data analysis, and portable device development will become important directions for the development of optical detection technology, further promoting its application in precision medicine and environmental monitoring.
Key words: Fluorescence detection / Fluorescent labeling / Fluorescence lifetime imaging microscopy / Fluorescence polarization immunoassay / Fluorescence resonance energy transfer
© The Author(s), published by EDP Sciences, 2026
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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