Issue |
J. Eur. Opt. Society-Rapid Publ.
Volume 20, Number 1, 2024
Plasmonica
|
|
---|---|---|
Article Number | 15 | |
Number of page(s) | 9 | |
DOI | https://doi.org/10.1051/jeos/2024015 | |
Published online | 29 April 2024 |
Research Article
Polarization-resolved surface-enhanced infrared spectra with nanosensors based on self-organized gold nanorods
1
Department of Physics, Sapienza University of Rome, P.le A. Moro 2, I-00185 Rome, Italy
2
Center for Life Nano-Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, I-00161 Rome, Italy
3
Dipartimento di Fisica, Università degli Studi di Genova, Via Dodecaneso 33, I-16146 Genova, Italy
* Corresponding author: raffaella.polito@uniroma1.it
Received:
31
January
2024
Accepted:
28
March
2024
Biosensors are becoming ubiquitous in the study of biomolecules, as, by modifying shape size and environment of metallic nanostructures it is now possible to engineer the field so to monitor subtle transient changes in molecular conformation at the level of a single biolayer. In this paper, we present a first step towards a polarization-resolved study of light-induced conformational changes of transmembrane proteins. We exploit a platform of self-organized gold nanorods on SiO2 substrates to enhance the infrared reflection absorption spectroscopy and to perform difference spectroscopy (i.e., spectrum under visible light ON minus spectrum under visible light OFF) on a light-sensitive transmembrane protein with simultaneous visible light illumination from the backside of the chip. The broad size distribution of nanorods allows us to probe with high sensitivity the modifications of the vibrational peaks over the entire fingerprint region. We show that it is possible to identify dissimilarities in the difference spectra, which in turn implies that we are monitoring over a broadband spectrum not only the chemical bonds with the dipole moment aligned orthogonally to our substrate/nanorod surface but also those with different orientation.
Key words: Self-organized nanoantennas / IR sensing / IRRAS / Near-field IR nanospectroscopy
© The Author(s), published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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