| Issue |
J. Eur. Opt. Society-Rapid Publ.
Volume 19, Number 1, 2023
Advancing Society with Light, a special issue from general congress ICO-25-OWLS-16-Dresden-Germany-2022
|
|
|---|---|---|
| Article Number | 31 | |
| Number of page(s) | 12 | |
| DOI | https://doi.org/10.1051/jeos/2023028 | |
| Published online | 08 June 2023 | |
Research Article
Brillouin scattering spectroscopy for studying human anatomy: Towards in situ mechanical characterization of soft tissue
1
Division of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, Austria
2
Medical Imaging Cluster (MIC), Medical University of Vienna, 1090 Vienna, Austria
3
Max Perutz Laboratories, University of Vienna, 1030 Vienna, Austria
* Corresponding author: kareem.elsayad@meduniwien.ac.at
Received:
27
March
2023
Accepted:
29
April
2023
Brillouin light scattering (BLS) spectroscopy is a label-free method of measuring the GHz-frequency viscoelastic properties. The measured longitudinal modulus is acutely sensitive to the degree of hydration, crosslinking, and temperature, which can be indicative of tissue health. As such, performing in situ measurements on humans is particularly desirable for exploring potential clinical translation, however, is not possible with existing designs which are coupled to bench-top microscopes. Here we introduce a robust fiber coupled hand-held BLS probe and demonstrate its reliability for measuring excised human tissue. We verify its accuracy using confocal BLS microscopy and further show that it is possible to distinguish veins, arteries, nerves and muscles based on their BLS-measured viscoelasticity. This provides a necessary first step towards in situ clinical BLS viscoelasticity studies of human tissue.
Key words: Brillouin light scattering spectroscopy / Hand-held optical probe / Viscoelasticity / Longitudinal modulus / Soft tissue / Muscles / Vasculature
© The Author(s), published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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