Issue |
J. Eur. Opt. Society-Rapid Publ.
Volume 19, Number 1, 2023
EOSAM 2022
|
|
---|---|---|
Article Number | 26 | |
Number of page(s) | 8 | |
DOI | https://doi.org/10.1051/jeos/2023022 | |
Published online | 24 May 2023 |
Research Article
Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope
1
Technische Universität Ilmenau, Fachgebiet Technische Optik, Postfach 100565, 98684 Ilmenau, Germany
2
Iba Heiligenstadt, 37308 Heilbad Heiligenstadt, Germany
* Corresponding author: meike.hofmann@tu-ilmenau.de
Received:
15
February
2023
Accepted:
19
April
2023
Light sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45° inclined tube. The element, which is based on a multi-focal diffractive lens and a linear grating, generates five thin light sheets with equal intensities when combined with a refractive cylindrical lens. The generated uniform light sheets can be applied for the scanning of samples in tubes enabling flow-driven 3-dimensional imaging.
Key words: Light sheet fluorescence microscopy / Diffractive optical elements / Multi-plane imaging / 3D imaging / Multi-focal lens
© The Author(s), published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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