Issue |
J. Eur. Opt. Soc.-Rapid Publ.
Volume 8, 2013
|
|
---|---|---|
Article Number | 13078 | |
Number of page(s) | 6 | |
DOI | https://doi.org/10.2971/jeos.2013.13078 | |
Published online | 12 December 2013 |
Regular papers
Label-free discrimination of cells undergoing apoptosis by hyperspectral confocual reflectance imaging
1
CNR-ISC Via del Fosso del Cavaliere 100, 00133 Roma, Italy
2
Dermatology Department, University of Tor Vergata, Viale Oxford 81, 00133 Roma, Italy
3
Dermatology Unit - NESMOS Department, Sapienza University of Rome, Italy
Received:
31
October
2013
Revised:
18
November
2013
Among the optical techniques used for exploring the properties of cells and tissues, those based on hyperspectral label-free analysis are particularly interesting due to their non-invasive character and their ability to fast collect a huge number of information on the different sample constituents and their spatial distribution. Here we present results obtained with a novel hyperspectral reflectance confocal microscope of label-free discrimination of cells undergoing apoptosis. Our data, analyzed by means of a powerful statistical method, enable to obtain information on the biological status at a single cell level through the local measurement of reflectivity. Furthermore, an optical model of the local dielectric response gives an additional insight of the parameters linking the optical responsivity to the biological status.
Key words: Confocal microscopy / hyperspectral imaging / spectroscopy / cell apoptosis / statistical methods
© The Author(s) 2013. All rights reserved.
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