application of the introduced pipeline to the simulated cells displayed in this work. Top row, slices from the simulated cells; Middle row, corresponding slices of the reconstructed simulated tomogram through the filtered backprojection routine. Bottom row, corresponding slices of the reconstructed simulated tomogram through the filtered backpropagation routine. Columns 1a and 1b refer to Simulation 1 with 1 and 7 cytoplasmic vacuoles respectively, columns 2, 3 and 4 refer to Simulation 2, 3 and 4. An equally spaced angular sequence was used, with the angles ranging from 0 to 360° with a step of 5°.