Issue |
J. Eur. Opt. Society-Rapid Publ.
Volume 20, Number 1, 2024
Plasmonica
|
|
---|---|---|
Article Number | 28 | |
Number of page(s) | 5 | |
DOI | https://doi.org/10.1051/jeos/2024028 | |
Published online | 02 July 2024 |
Short Communication
Towards a portable setup for the on-site SERS detection of miRNAs
Politecnico di Torino, c.so Duca degli Abruzzi 24, 10129 Torino, Italy
* Corresponding author: chiara.novara@polito.it
Received:
5
April
2024
Accepted:
17
May
2024
The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by surface enhanced Raman scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.
Key words: Surface enhanced Raman scattering / miRNA / Microfluidic setup / On-site Raman measurements
© The Author(s), published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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