Issue |
J. Eur. Opt. Soc.-Rapid Publ.
Volume 8, 2013
|
|
---|---|---|
Article Number | 13075 | |
Number of page(s) | 6 | |
DOI | https://doi.org/10.2971/jeos.2013.13075 | |
Published online | 03 December 2013 |
Regular papers
Optical characterization of aminosilane-modified silicon dioxide surface for biosensing
1
Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, I-80131 Naples, Italy
2
Dept. of Pharmacy, University of Naples Federico II, Naples, Italy
3
Dept. of Chemical Sciences - “Federico II” University of Naples, Naples Italy
Received:
23
September
2013
Revised:
12
November
2013
Silicon dioxide surfaces, functionalized by two aminosilane compounds (3-amino-propyl-triethoxysilane, APTES; 3-amino-propyl-dimethyl-ethoxysilane, APDMES) both dissolved in different solvents (dry ethanol and toluene), have been investigated by standard techniques such as spectroscopic ellipsometry (SE), water contact angle (WCA), and atomic force microscopy (AFM). Silane thicknesses between 5 and 80 Å have been found, depending on deposition conditions; surface wettabilities change, accordingly. These organic-inorganic interfaces have also been modified by a cross-linker (bis-sulfosuccinimidyl suberate) in order to covalently bind a fluorescein labeled protein A. The amount of protein linked to functional surfaces has been quantified by SE and fluorescence microscopy. These results could be very useful in developing new platforms for optical biosensing.
Key words: Surface functionalization / biomaterials / ellipsometry / water contact angle / atomic force microscopy
© The Author(s) 2013. All rights reserved.
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