Issue |
J. Eur. Opt. Soc.-Rapid Publ.
Volume 4, 2009
|
|
---|---|---|
Article Number | 09024 | |
Number of page(s) | 5 | |
DOI | https://doi.org/10.2971/jeos.2009.09024 | |
Published online | 01 June 2009 |
Regular papers
Magnetite nanoparticles for biosensor model based on bacteria fluorescence
1
University of Medicine and Pharmacy, Iasi, Romania
2
University ”Al. I. Cuza”, Fac. of Physics, Iasi, Romania
3
Institute of Macromolecular Chemistry ”P.Poni”, Iasi, Romania
Received:
30
September
2008
Fluorescence emission of pyoverdine – the siderophore synthesized by iron scavenger bacteria – was studied using in vitro cultures of Pseudomonas aeruginosa with the aim to design a biosensor system for liquid sample iron loading. Diluted suspensions of colloidal magnetite nanoparticles were supplied in the culture medium (10 microl/l and 100 microl/l) to simulate magnetic loading with iron oxides of either environmental waters or human body fluids. The electromagnetic exposure to radiofrequency waves of bacterial samples grown in the presence of magnetic nanoparticles was also carried out. Cell density diminution but fluorescence stimulation following 10 microl/l ferrofluid addition and simultaneous exposure to radiofrequency waves was evidenced. The inhibitory influence of 100 microl/l ferrofluid combined with RF exposure was evidenced by fluorescence data. Mathematical model was proposed to approach quantitatively the dynamics of cell density and fluorescence emission in relation with the consumption of magnetite nanoparticle supplied medium. The biosensor scheme was shaped based on the response to iron loading of bacterial sample fluorescence.
Key words: colloidal magnetite / iron chelate / iron scavenger bacteria
© The Author(s) 2009. All rights reserved.
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