Representative DS profile for a 30 mm source–detector separation with a 500 μm diameter pore (positioned at 0.5 mm off-axis relative to the optical source center). The DS curves obtained across all individual cadaveric models and pore positions were found to be virtually identical, following the same spatial distribution. For visual clarity, this single representative profile is shown to illustrate the system’s sensitivity to underlying tissue volumes, which remains stable despite surface-level intensity fluctuations.