Issue |
J. Eur. Opt. Soc.-Rapid Publ.
Volume 4, 2009
|
|
---|---|---|
Article Number | 09028 | |
Number of page(s) | 5 | |
DOI | https://doi.org/10.2971/jeos.2009.09028 | |
Published online | 03 June 2009 |
Regular papers
Marker-free cell discrimination by holographic optical tweezers
1
University of Stuttgart, Institut für Technische Optik, Stuttgart, 70569, Germany
2
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology and University of Tuebingen, 70376, Germany
Received:
28
November
2008
We introduce a method for marker-free cell discrimination based on optical tweezers. Cancerous, non-cancerous, and drug-treated cells could be distinguished by measuring the trapping forces using holographic optical tweezers. We present trapping force measurements on different cell lines: normal pre-B lymphocyte cells (BaF3; “normal cells”), their Bcr-Abl transformed counterparts (BaF3-p185; “cancer cells”) as a model for chronic myeloid leukaemia (CML) and Imatinib treated BaF3-p185 cells. The results are compared with reference measurements obtained by a commercial flow cytometry system.
Key words: optical tweezers / marker free cell discrimination / chronic myeloid leukaemia
© The Author(s) 2009. All rights reserved.
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